Zinc oxide nanorods functionalized paper for protein preconcentration in biodiagnostics
نویسندگان
چکیده
Distinguishing a specific biomarker from a biofluid sample containing a large variety of proteins often requires the selective preconcentration of that particular biomarker to a detectable level for analysis. Low-cost, paper-based device is an emerging opportunity in diagnostics. In the present study, we report a novel Zinc oxide nanorods functionalized paper platform for the preconcentration of Myoglobin, a cardiac biomarker. Zinc oxide nanorods were grown on a Whatman filter paper no. 1 via the standard hydrothermal route. The growth of Zinc oxide nanorods on paper was confirmed by a combination of techniques consisting of X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS,) scanning electron microscopy (SEM), and energy dispersive spectroscopy (EDX) analysis. The Zinc oxide nanorods modified Whatman filter paper (ZnO-NRs/WFP) was further tested for use as a protein preconcentrator. Paper-based ELISA was performed for determination of pre-concentration of cardiac marker protein Myoglobin using the new ZnO-NRs/WFP platform. The ZnO-NRs/WFP could efficiently capture the biomarker even from a very dilute solution (Myoglobin < 50 nM). Our ELISA results show a threefold enhancement in protein capture with ZnO-NRs/WFP compared to unmodified Whatman filter paper, allowing accurate protein analysis and showing the diagnostic concept.
منابع مشابه
Effect of Seed Layer on the Morphology of Zinc Oxide Nanorods as an Electron Transport Layer in Polymer Solar Cells
Zinc oxide has been considered as a promising semiconductor material for fabrication of transparent conductive oxides (TCOs), electronic devices, optoelectronics, and solar cells. Among the various morphologies of zinc oxide, nanorods are more widely used because of the ease of synthesis and providing a direct path for the transport of charge carriers. The electrochemical deposi...
متن کاملFabrication of Resistive E. coli Biosensor Based on ZnO Nanorods and Nanoparticles
In this paper, a biosensor has been fabricated using ZnO nanorods and nanoparticles to detect different concentrations of the E. coli bacteria. The innovation of this paper lies in design and fabrication of the resistive type E Coli bacteria sensor. To make this biosensors, printed circuit board based electrodes are designed and made in an interdigitated shape. Both hydrothermal and drop cast m...
متن کاملIron Ion Sensor Based on Functionalized ZnO Nanorods, 2012, Electroanalysis
In this work, we are presenting an iron ion (Fe 3+ ) potentiometric sensor based on functionalized ZnO nanorods with selective ionophore (18 crown 6). Zinc oxide nanorods with a diameter of about 100 to 150 nm and 1μm in length were grown on gold coated glass. The selective Fe 3+ ionophore sensor with highly aligned ZnO nanorods has given high sensitivity, acceptable selectivity, reproducibilit...
متن کاملZnO Nanostructure-Based Intracellular Sensor
Recently ZnO has attracted much interest because of its usefulness for intracellular measurements of biochemical species by using its semiconducting, electrochemical, catalytic properties and for being biosafe and biocompatible. ZnO thus has a wide range of applications in optoelectronics, intracellular nanosensors, transducers, energy conversion and medical sciences. This review relates specif...
متن کاملZnO Quasi-1D Nanostructures: Synthesis, Modeling, and Properties for Applications in Conductometric Chemical Sensors
One-dimensional metal oxide nanostructures such as nanowires, nanorods, nanotubes, and nanobelts gained great attention for applications in sensing devices. ZnO is one of the most studied oxides for sensing applications due to its unique physical and chemical properties. In this paper, we provide a review of the recent research activities focused on the synthesis and sensing properties of pure,...
متن کامل